ARG57416
anti-Estrogen related Receptor alpha antibody
anti-Estrogen related Receptor alpha antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Estrogen related Receptor alpha |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Estrogen related Receptor alpha |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-423 of Human Estrogen related Receptor alpha (NP_004442.3). |
| 偶联标记 | Un-conjugated |
| 別名 | ERRalpha; ERRa; NR3B1; ESRL1; Nuclear receptor subfamily 3 group B member 1; Estrogen-related receptor alpha; ERR-alpha; Steroid hormone receptor ERR1; Estrogen receptor-like 1; ERR1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | 293T | ||||||||
| 实际分子量 | ~ 52 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ESRRA |
| 全名 | estrogen-related receptor alpha |
| 背景介绍 | The protein encoded by this gene is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site-specific transcription regulator and has been also shown to interact with estrogen and the transcripton factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium-chain acyl coenzyme A dehydrogenase (MCAD) and thyroid hormone receptor genes. A processed pseudogene of ESRRA is located on chromosome 13q12.1. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2013] |
| 生物功能 | Binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism. Induces the expression of PERM1 in the skeletal muscle. [UniProt] |
| 预测分子量 | 46 kDa |
| 翻译后修饰 | Phosphorylation on Ser-19 enhances sumoylation on Lys-14 increasing repression of transcriptional activity. Sumoylated with SUMO2. Main site is Lys-14 which is enhanced by phosphorylation on Ser-19, cofactor activation, and by interaction with PIAS4. Sumoylation enhances repression of transcriptional activity, but has no effect on subcellular location nor on DNA binding. Reversibly acetylated. Acetylation by PCAF/KAT2 at Lys-129, Lys-138, Lys-160 and Lys-162 and PCAF/KAT2 decreases transcriptional activity probably by inhibiting DNA-binding activity; deacetylation involves SIRT1 and HDAC8 and increases DNA-binding. |
检测图片 (3) Click the Picture to Zoom In
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ARG57416 anti-Estrogen related Receptor alpha antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG57416 anti-Estrogen related Receptor alpha antibody at 1:100 dilution.
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ARG57416 anti-Estrogen related Receptor alpha antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human uterus tissue stained with ARG57416 anti-Estrogen related Receptor alpha antibody at 1:200 dilution.
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ARG57416 anti-Estrogen related Receptor alpha antibody WB image
Western blot: 25 µg of 293T cell lysate stained with ARG57416 anti-Estrogen related Receptor alpha antibody at 1:1000 dilution.
