ARG65738
anti-Estrogen Receptor beta antibody
anti-Estrogen Receptor beta antibody for Western blot and Human
Cancer antibody; Gene Regulation antibody; Neuroscience antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes Estrogen Receptor beta |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 特异性 | This antibody is expected to recognize all reported isoforms (NP_001428.1; NP_001035365.1; NP_001201831.1; NP_001201832.1). Reported variants represent identical protein: NP_001035365.1, NP_001035366.1 |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Estrogen Receptor beta |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide from the Center region of Human Estrogen Receptor beta. (C-TQDADSSRKLAH) |
| 偶联标记 | Un-conjugated |
| 別名 | ER-BETA; NR3A2; ESTRB; Estrogen receptor beta; ER-beta; ESRB; Erb; Nuclear receptor subfamily 3 group A member 2; ESR-BETA |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ESR2 |
| 全名 | estrogen receptor 2 (ER beta) |
| 背景介绍 | This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008] |
| 生物功能 | Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. [UniProt] |
| 研究领域 | Cancer antibody; Gene Regulation antibody; Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 53 kDa (NP_001035366.1) |
| 翻译后修饰 | Phosphorylation at Ser-87 and Ser-105 recruits NCOA1. |
检测图片 (1) Click the Picture to Zoom In
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ARG65738 anti-Estrogen Receptor beta antibody WB image
Western blot: 35 µg of Human Placenta lysate stained with ARG65738 anti-Estrogen Receptor beta antibody at 1 µg/ml dilution (1h incubation).
