ARG51684
anti-Ephrin B2 phospho (Tyr330) antibody
anti-Ephrin B2 phospho (Tyr330) antibody for Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Neuroscience antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Ephrin B2 phospho (Tyr330) |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Ephrin B2 |
| 抗原物种 | Human |
| 抗原 | Peptide sequence around phosphorylation site of tyrosine 330 (N-I-Y(p)-Y-K) derived from Human Ephrin-B2 |
| 偶联标记 | Un-conjugated |
| 別名 | HTK ligand; HTKL; Htk-L; EPLG5; Ephrin-B2; HTK-L; LERK5; LERK-5; EPH-related receptor tyrosine kinase ligand 5 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic peptide. Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
| 缓冲液 | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | EFNB2 |
| 全名 | ephrin-B2 |
| 背景介绍 | Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. |
| 生物功能 | Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons. [UniProt] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Neuroscience antibody |
| 预测分子量 | 37 kDa |
| 翻译后修饰 | Inducible phosphorylation of tyrosine residues in the cytoplasmic domain. |
检测图片 (1) Click the Picture to Zoom In
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ARG51684 anti-Ephrin B2 phospho (Tyr330) antibody WB image
Western blot: Extracts from HT29 cells, untreated or treated with UV stained with ARG51684 anti-Ephrin B2 phospho (Tyr330) antibody.
