anti-EpCAM antibody [SQab18103]

anti-EpCAM antibody [SQab18103] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Controls and Markers antibody; Epithelial Marker antibody; Circulating Tumor Cells BioMarker antibody


产品描述 Recombinant Rabbit Monoclonal antibody [SQab18103] recognizes EpCAM
反应物种 Hu
应用 IHC-P, WB
宿主 Rabbit
克隆 Monoclonal
克隆号 SQab18103
同位型 IgG
靶点名称 EpCAM
抗原物种 Human
抗原 Synthetic peptide within aa. 1-100 of Human EpCAM.
偶联标记 Un-conjugated
別名 MIC18; EGP; Tumor-associated calcium signal transducer 1; Epithelial glycoprotein 314; KSA; Ep-CAM; Epithelial cell surface antigen; Adenocarcinoma-associated antigen; HNPCC8; Cell surface glycoprotein Trop-1; EGP40; TACSTD1; KS1/4; hEGP314; Major gastrointestinal tumor-associated protein GA733-2; M4S1; MK-1; Epithelial glycoprotein; KS 1/4 antigen; ESA; DIAR5; EGP314; Epithelial cell adhesion molecule; EGP-2; TROP1; CD antigen CD326


应用 推荐稀释比
IHC-P1:100 - 1:200
WB1:500 - 1:4000
应用说明 IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT for 30 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 35 kDa


形式 Liquid
纯化 Purification with Protein A.
缓冲液 PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂 0.01% Sodium azide
稳定剂 40% Glycerol and 0.05% BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.



GeneID: 4072 Human EPCAM

Swiss-port # P16422 Human Epithelial cell adhesion molecule

基因名称 EPCAM
全名 epithelial cell adhesion molecule
背景介绍 EpCAM is a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
生物功能 EpCAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. [UniProt]
产品亮点 Related products:
EpCAM antibodies; EpCAM ELISA Kits; EpCAM Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Cancer Pathology Markers (SQ clones)
研究领域 Controls and Markers antibody; Epithelial Marker antibody; Circulating Tumor Cells BioMarker antibody
预测分子量 35 kDa
翻译后修饰 Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability. [UniProt]

检测图片 (2) Click the Picture to Zoom In

  • ARG66353 anti-EpCAM antibody [SQab18103] WB image (Customer's Feedback)

    Western blot: HT-29 cell lysate stained with ARG66353 anti-EpCAM antibody [SQab18103] at 1:1000 dilution.

  • ARG66353 anti-EpCAM antibody [SQab18103] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded colon tissue stained with ARG66353 anti-EpCAM antibody [SQab18103]. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).


nuts_pic      Excellent

anti-EpCAM antibody [SQab18103]



Sample Loading Amount:20 μg

SDS Page Type:Denatured

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:HRP