ARG55159

Name: anti-Enolase 1 antibody
Brand: arigo Biolaboratories
SKU: ARG55159
Price: 399 USD
Availability: InStock
Rating: 5.0 (1 reviews)

anti-Enolase 1 antibody

Datasheet

订购信息

包装

价格 (USD)

100 μl

399

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Rabbit Polyclonal antibody recognizes Enolase 1
反应物种	Hu, Ms, Rat
预测物种	Bov, Rat, Chk, Mk, Xenopus
应用	FACS, ICC/IF, IHC-P, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	Enolase 1
抗原物种	Human
抗原	KLH-conjugated synthetic peptide corresponding to aa. 178-205 (Center) of Human Enolase 1.
偶联标记	Un-conjugated
別名	MPB1; Plasminogen-binding protein; Alpha-enolase; MBP-1; NNE; PPH; Enolase 1; ENO1L1; Phosphopyruvate hydratase; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Non-neural enolase; MPB-1; EC 4.2.1.11

应用说明

应用建议

应用	推荐稀释比
FACS	1:10 - 1:50
ICC/IF	1:10 - 1:50
IHC-P	Assay-dependent
WB	1:1000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
缓冲液	PBS and 0.09% (W/V) Sodium azide
抗菌剂	0.09% (W/V) Sodium azide
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 13806 Mouse ENO1 GeneID: 2023 Human ENO1 GeneID: 24333 Rat ENO1 Swiss-port # P04764 Rat Alpha-enolase Swiss-port # P06733 Human Alpha-enolase Swiss-port # P17182 Mouse Alpha-enolase More
基因名称	ENO1
全名	enolase 1, (alpha)
背景介绍	This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]
生物功能	Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor. [UniProt]
细胞定位	Cytoplasm. Cell membrane. Cytoplasm, myofibril, sarcomere, M line. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line
研究领域	Cancer antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody
预测分子量	47 kDa
翻译后修饰	ISGylated.

检测图片 (4) Click the Picture to Zoom In

ARG55159 anti-Enolase 1 antibody WB image

Western blot: 30 µg of HeLa, Jurkat , HepG2, DU145, MDAMB231, HEK293T, Mouse heart, Mouse liver, Rat heart and Rat liver lysates stained with ARG55159 anti-Enolase 1 antibody at 1:500 dilution.
ARG55159 anti-Enolase 1 antibody ICC/IF image

Immunofluorescence: C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then stained with ARG55159 anti-Enolase 1 antibody (green) at 1:25 dilution, 1 hour at 37°C. Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml, 1 hour at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).
ARG55159 anti-Enolase 1 antibody IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded Human lymph stained with ARG55159 anti-Enolase 1 antibody.
ARG55159 anti-Enolase 1 antibody FACS image

Flow Cytometry: HeLa cells stained with ARG55159 anti-Enolase 1 antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with FITC labelled secondary antibody.