ARG55749
anti-Enolase 1 antibody [786CT6.6.4]
anti-Enolase 1 antibody [786CT6.6.4] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
概述
| 产品描述 | Mouse Monoclonal antibody recognizes Enolase 1 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 786CT6.6.4 |
| 同位型 | IgG, kappa |
| 靶点名称 | Enolase 1 |
| 抗原物种 | Human |
| 抗原 | Purified His-tagged Human Enolase1 protein |
| 偶联标记 | Un-conjugated |
| 別名 | MPB1; Plasminogen-binding protein; Alpha-enolase; MBP-1; NNE; PPH; Enolase 1; ENO1L1; Phosphopyruvate hydratase; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Non-neural enolase; MPB-1; EC 4.2.1.11 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | NIH/3T3 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ENO1 |
| 全名 | enolase 1, (alpha) |
| 背景介绍 | This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011] |
| 生物功能 | Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor. [UniProt] |
| 细胞定位 | Cytoplasm. Cell membrane. Cytoplasm, myofibril, sarcomere, M line. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line |
| 预测分子量 | 47 kDa |
| 翻译后修饰 | ISGylated. |
检测图片 (2) Click the Picture to Zoom In
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ARG55749 anti-Enolase 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human kideny tissue stained with ARG55749 anti-Enolase 1 antibody at 1:25 dilution.
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ARG55749 anti-Enolase 1 antibody WB image
Western blot: 35 µg of NIH/3T3 cell lysate stained with ARG55749 anti-Enolase 1 antibody.
