ARG53992
anti-Eg 5 antibody
anti-Eg 5 antibody for ICC/IF,Immunoprecipitation,Western blot and Human
Gene Regulation antibody; Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody recognizes Eg 5 |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, IP, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG1 |
| 靶点名称 | Eg 5 |
| 抗原物种 | Human |
| 抗原 | Purified recombinant human Eg5 protein fragments expressed in E.coli. |
| 偶联标记 | Un-conjugated |
| 別名 | TRIP-5; Kinesin-related motor protein Eg5; KNSL1; TR-interacting protein 5; HKSP; Kinesin-like spindle protein HKSP; EG5; MCLMR; Kinesin-like protein 1; Kinesin-like protein KIF11; TRIP5; Thyroid receptor-interacting protein 5 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 130 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | KIF11 |
| 全名 | kinesin family member 11 |
| 背景介绍 | Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays. |
| 生物功能 | Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays. [UniProt] |
| 细胞定位 | Cytoplasm |
| 研究领域 | Gene Regulation antibody; Signaling Transduction antibody |
| 预测分子量 | 119 kDa |
| 翻译后修饰 | Phosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase). A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function. |
检测图片 (3) Click the Picture to Zoom In
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ARG53992 anti-Eg 5 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG53992 anti-Eg 5 antibody at 1:200 dilution.
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ARG53992 anti-Eg 5 antibody WB image
Western blot: MCF7 cell lysate stained with ARG53992 anti-Eg 5 antibody at 1:1000 dilution.
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ARG53992 anti-Eg 5 antibody IP image
Immunoprecipitation: Jurkat cell lysates were immunoprecipitated and stained with ARG53992 anti-Eg 5 antibody.
