ARG54700
anti-EZH2 antibody [144CT2.1.1.5]
anti-EZH2 antibody [144CT2.1.1.5] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Gene Regulation antibody; Polycomb Complexes antibody
概述
| 产品描述 | Mouse Monoclonal antibody recognizes EZH2 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Mk |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 144CT2.1.1.5 |
| 同位型 | IgG1 Kappa |
| 靶点名称 | EZH2 |
| 抗原 | EZH2 recombinant protein. |
| 偶联标记 | Un-conjugated |
| 別名 | ENX-1; Lysine N-methyltransferase 6; KMT6; ENX1; WVS2; WVS; Enhancer of zeste homolog 2; EZH2b; Histone-lysine N-methyltransferase EZH2; EZH1; EC 2.1.1.43; KMT6A |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 纯化 | Protein G purified |
|---|---|
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q15910 Human Histone-lysine N-methyltransferase EZH2 |
|---|---|
| 基因名称 | EZH2 |
| 全名 | enhancer of zeste 2 polycomb repressive complex 2 subunit |
| 背景介绍 | This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Feb 2011] |
| 生物功能 | Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys- 27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription. [From Uniprot] |
| 细胞定位 | Nucleus |
| 研究领域 | Cancer antibody; Gene Regulation antibody; Polycomb Complexes antibody |
| 预测分子量 | 85 kDa |
| 翻译后修饰 | Phosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity. Phosphorylation at Thr-345 by CDK1 and CDK2 promotes maintenance of H3K27me3 levels at EZH2-target loci, thus leading to epigenetic gene silencing. Sumoylated. Glycosylated: O-GlcNAcylation at Ser-75 by OGT increases stability of EZH2 and facilitates the formation of H3K27me3 by the PRC2/EED-EZH2 complex. |
检测图片 (3) Click the Picture to Zoom In
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ARG54700 anti-EZH2 antibody WB image
Western blot: 20 µg of SW480 and HCT116 cell lysates stained with ARG54700 anti-EZH2 antibody at 1:100 dilution.
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ARG54700 anti-EZH2 antibody ICC/IF image
Immunofluorescence: U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then stained with ARG54700 anti-EZH2 antibody (1:25, 1 h at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C).
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ARG54700 anti-EZH2 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human testis tissue stained with ARG54700 anti-EZH2 antibody.
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anti-EZH2 antibody [144CT2.1.1.5]
Application：WB
Sample：SW480 and HCT116
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:100
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
