ARG55271
anti-EZH1 antibody
anti-EZH1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Gene Regulation antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes EZH1 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Rat |
| 应用 | IHC-P, WB |
| 特异性 | Multiple isoforms of EZH1 are known to exist. EZH1 antibody is predicted to not cross-react with EZH2 |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | EZH1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide (18 aa) within aa. 180-230 of Human EZH1. |
| 偶联标记 | Un-conjugated |
| 別名 | Enhancer of zeste homolog 1; ENX-2; EC 2.1.1.43; KMT6B; Histone-lysine N-methyltransferase EZH1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Mouse Lung Tissue Lysate |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P70351 Mouse Histone-lysine N-methyltransferase EZH1 Swiss-port # Q92800 Human Histone-lysine N-methyltransferase EZH1 |
|---|---|
| 基因名称 | EZH1 |
| 全名 | enhancer of zeste 1 polycomb repressive complex 2 subunit |
| 背景介绍 | EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 (see MIM 602812) lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity (Shen et al., 2008 [PubMed 19026780]).[supplied by OMIM, Mar 2009] |
| 生物功能 | Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH2-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. [UniProt] |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 85 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG55271 anti-EZH1 antibody WB image
Western blot: Mouse lung tissue lysate stained with ARG55271 anti-EZH1 antibody at (A) 1 and (B) 2 µg/ml dilution.
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ARG55271 anti-EZH1 antibody IHC image
Immunohistochemistry: Human lung tissue stained with ARG55271 anti-EZH1 antibody at 20 µg/ml dilution.
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ARG55271 anti-EZH1 antibody IHC-P image
Immunohistochemistry: Human lung tissue stained with ARG55271 anti-EZH1 antibody at 5 µg/ml dilution.
