ARG45559
anti-EXOSC3 antibody
anti-EXOSC3 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes EXOSC3 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | EXOSC3 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human EXOSC3. |
| 偶联标记 | Un-conjugated |
| 別名 | EXOSC3; Exosome Component 3; RRP40; P10; CGI-102; HRrp-40; Rrp40p; Ribosomal RNA-Processing Protein 40; Exosome Complex Component RRP40; HRrp40p; Exosome Complex Exonuclease RRP40; Exosome Component Rrp40; CGI-102 Protein; BA3J10.7; PCH1B |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 实际分子量 | 30 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | EXOSC3 |
| 全名 | Exosome Component 3 |
| 背景介绍 | This gene encodes a non-catalytic component of the human exosome, a complex with 3'-5' exoribonuclease activity that plays a role in numerous RNA processing and degradation activities. Related pseudogenes of this gene are found on chromosome 19 and 21. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2012] |
| 生物功能 | Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC3 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of RNase PH-domain subunits through contacts with EXOSC9 and EXOSC5. [UniProt] |
| 细胞定位 | Cytoplasm; Exosome; Nucleus. [UniProt] |
| 预测分子量 | 30 kDa |
| 翻译后修饰 | Acetylation; Isopeptide bond; Ubl conjugation. [UniProt] |
检测图片 (5) Click the Picture to Zoom In
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ARG45559 anti-EXOSC3 antibody IHC-P image
Immunohistochemistry: Human gall bladder adenosquamous carcinoma stained with ARG45559 anti-EXOSC3 antibody at 2 μg/ml dilution.
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ARG45559 anti-EXOSC3 antibody ICC/IF image
Immunofluorescence: A431 stained with ARG45559 anti-EXOSC3 antibody at 5 μg/ml dilution.
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ARG45559 anti-EXOSC3 antibody WB image
Western blot: HEK293 stained with ARG45559 anti-EXOSC3 antibody at 0.5 μg/ml dilution.
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ARG45559 anti-EXOSC3 antibody FACS image
Flow Cytometry: PC-3 stained with ARG45559 anti-EXOSC3 antibody at 1 µg/10^6 cells dilution.
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ARG45559 anti-EXOSC3 antibody WB image
Western blot: RAW264.7 stained with ARG45559 anti-EXOSC3 antibody at 0.5 μg/ml dilution.
