ARG64475
anti-EXO1 antibody
anti-EXO1 antibody for Western blot and Human
Gene Regulation antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes EXO1 |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 特异性 | This antibody is expected to recognise all three reported isoforms (NP_003677.3; NP_006018.3 and NP_569082.1) |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | EXO1 |
| 抗原物种 | Human |
| 抗原 | C-HRNYSPRPESGT |
| 偶联标记 | Un-conjugated |
| 別名 | Exonuclease I; hExoI; EC 3.1.-.-; hExo1; HEX1; Exonuclease 1 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | This gene encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms. [provided by RefSeq, Jul 2008] |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 94 kDa |
| 翻译后修饰 | Phosphorylated upon DNA damage and in response to agents stalling DNA replication, probably by ATM or ATR. Phosphorylation at Ser-454, Thr-621 and Ser-714 is induced upon DNA-damage caused by treatment with hydroxyurea (HU) but not upon IR treatment. The HU-induced EXO1 triple phosphorylation facilitates destabilisation/degradation of the protein. |
检测图片 (1) Click the Picture to Zoom In
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ARG64475 anti-EXO1 antibody WB image
Western Blot: A549 cell lysate (35 µg protein in RIPA buffer) stained with ARG64475 anti-EXO1 antibody at 0.2 µg/ml dilution.
