ARG65286

anti-ETFB antibody

anti-ETFB antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cancer antibody; Metabolism antibody; Signaling Transduction antibody

概述

产品描述 Goat Polyclonal antibody recognizes ETFB
反应物种 Hu, Ms, Rat
预测物种 Cow, Dog
应用 IHC-P, WB
特异性 This antibody is expected to recognize both reported isoforms (NP_001976.1; NP_001014763.1). Amino acid numbering in the name above refers to NP_001976.1).
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 ETFB
抗原物种 Human
抗原 C-QVTLEGDKLKVERE
偶联标记 Un-conjugated
別名 MADD; FP585; Beta-ETF; Electron transfer flavoprotein subunit beta

应用说明

应用建议
应用 推荐稀释比
IHC-P3 - 5 µg/ml
WB1 - 3 µg/ml
应用说明 IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified from goat serum by antigen affinity chromatography.
缓冲液 Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂 0.02% Sodium azide
稳定剂 0.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 110826 Mouse ETFB

GeneID: 2109 Human ETFB

GeneID: 292845 Rat ETFB

背景介绍 This gene encodes electron-transfer-flavoprotein, beta polypeptide, which shuttles electrons between primary flavoprotein dehydrogenases involved in mitochondrial fatty acid and amino acid catabolism and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. The gene deficiencies have been implicated in type II glutaricaciduria. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]
研究领域 Cancer antibody; Metabolism antibody; Signaling Transduction antibody
预测分子量 28 kDa
翻译后修饰 Methylated. Trimethylation at Lys-200 and Lys-203 may negatively regulate the activity in electron transfer from Acyl-CoA dehydrogenases.

检测图片 (2) Click the Picture to Zoom In

  • ARG65286 anti-ETFB antibody WB image

    Western blot: Human Liver lysate (35 µg protein in RIPA buffer) stained with ARG65286 anti-ETFB antibody at 0.1 µg/ml dilution.

  • ARG65286 anti-ETFB antibody IHC-P image

    Immunohistochemistry: Paraffin embedded Human Liver. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG65286 anti-ETFB antibody at 3.8 µg/ml dilution followed by AP-staining.