ARG55364
anti-ETFA antibody
anti-ETFA antibody for ICC/IF,Western blot and Human,Mouse
Cancer antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ETFA |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ETFA |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 192-226 (Center) of Human ETFA. |
| 偶联标记 | Un-conjugated |
| 別名 | Electron transfer flavoprotein subunit alpha, mitochondrial; MADD; EMA; GA2; Alpha-ETF |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | A431 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P13804 Human Electron transfer flavoprotein subunit alpha, mitochondrial Swiss-port # Q99LC5 Mouse Electron transfer flavoprotein subunit alpha, mitochondrial |
|---|---|
| 基因名称 | ETFA |
| 全名 | electron-transfer-flavoprotein, alpha polypeptide |
| 背景介绍 | ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). [UniProt] |
| 细胞定位 | Mitochondrion matrix. |
| 研究领域 | Cancer antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 35 kDa |
| 翻译后修饰 | The N-terminus is blocked. |
检测图片 (2) Click the Picture to Zoom In
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ARG55364 anti-ETFA antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG55364 anti-ETFA antibody (green) at 1:25 dilution. Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red). DAPI (blue) for nuclear staining.
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ARG55364 anti-ETFA antibody WB image
Western blot: 35 µg of A431 cell lysate stained with ARG55364 anti-ETFA antibody at 1:1000 dilution.
