ARG66770
anti-ERG antibody [SQab20203]
anti-ERG antibody [SQab20203] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Recombinant Rabbit Monoclonal antibody [SQab20203] recognizes ERG |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SQab20203 |
| 同位型 | IgG |
| 靶点名称 | ERG |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide within aa. 450 to C-terminus of Human ERG. |
| 偶联标记 | Un-conjugated |
| 別名 | p55; Transcriptional regulator ERG; Transforming protein ERG; erg-3 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | Appendix tissue; Molt-4 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ERG |
| 全名 | v-ets avian erythroblastosis virus E26 oncogene homolog |
| 背景介绍 | This gene encodes a member of the erythroblast transformation-specific (ETS) family of transcriptions factors. All members of this family are key regulators of embryonic development, cell proliferation, differentiation, angiogenesis, inflammation, and apoptosis. The protein encoded by this gene is mainly expressed in the nucleus. It contains an ETS DNA-binding domain and a PNT (pointed) domain which is implicated in the self-association of chimeric oncoproteins. This protein is required for platelet adhesion to the subendothelium, inducing vascular cell remodeling. It also regulates hematopoesis, and the differentiation and maturation of megakaryocytic cells. This gene is involved in chromosomal translocations, resulting in different fusion gene products, such as TMPSSR2-ERG and NDRG1-ERG in prostate cancer, EWS-ERG in Ewing's sarcoma and FUS-ERG in acute myeloid leukemia. More than two dozens of transcript variants generated from combinatorial usage of three alternative promoters and multiple alternative splicing events have been reported, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Apr 2014] |
| 生物功能 | Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure. [UniProt] |
| 细胞定位 | Nucleus. Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. [UniProt] |
| 预测分子量 | 55 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG66770 anti-ERG antibody [SQab20203] WB image (Customer's Feedback)
Western blot: Molt-4 cell lysate stained with ARG66770 anti-ERG antibody [SQab20203] at 1:1000 dilution.
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ARG66770 anti-ERG antibody [SQab20203] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human appendix tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66770 anti-ERG antibody [SQab20203] at 18°C - 25°C for 30 minutes.
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anti-ERG antibody [SQab20203]
Application：WB
Sample：Molt-4
Sample Loading Amount：20 μg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：Overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：HRP
