ARG58392
anti-ERCC8 antibody
anti-ERCC8 antibody for Western blot and Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ERCC8 |
|---|---|
| 反应物种 | Ms |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ERCC8 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide within aa. 300 to the C-terminus of Human ERCC8 (NP_000073.1). |
| 偶联标记 | Un-conjugated |
| 別名 | DNA excision repair protein ERCC-8; CSA; CKN1; UVSS2; Cockayne syndrome WD repeat protein CSA |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | Mouse testis | ||||
| 实际分子量 | 44 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q8CFD5 Mouse DNA excision repair protein ERCC-8 |
|---|---|
| 基因名称 | ERCC8 |
| 全名 | excision repair cross-complementation group 8 |
| 背景介绍 | This gene encodes a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). CS cells are abnormally sensitive to ultraviolet radiation and are defective in the repair of transcriptionally active genes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014] |
| 生物功能 | Substrate-recognition component of the CSA complex, a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, involved in transcription-coupled nucleotide excision repair. The CSA complex (DCX(ERCC8) complex) promotes the ubiquitination and subsequent proteasomal degradation of ERCC6 in a UV-dependent manner; ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair. It is required for the recruitment of XAB2, HMGN1 and TCEA1/TFIIS to a transcription-coupled repair complex which removes RNA polymerase II-blocking lesions from the transcribed strand of active genes. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 44 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG58392 anti-ERCC8 antibody WB image
Western blot: 25 µg of Mouse testis lysate stained with ARG58392 anti-ERCC8 antibody at 1:1000 dilution.
