ARG62480
anti-ERCC1 antibody [3H11]
anti-ERCC1 antibody [3H11] for Immunoprecipitation,Western blot and Human
Cancer antibody; Gene Regulation antibody
概述
| 产品描述 | Mouse Monoclonal antibody [3H11] recognizes ERCC1 |
|---|---|
| 反应物种 | Hu |
| 应用 | IP, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 3H11 |
| 同位型 | IgG2a |
| 靶点名称 | ERCC1 |
| 抗原物种 | Human |
| 抗原 | His-tagged recombinant human ERCC1 protein. |
| 偶联标记 | Un-conjugated |
| 別名 | DNA excision repair protein ERCC-1; RAD10; COFS4; UV20 |
应用说明
| 应用说明 | WB: 1/375 - 1/750 IP: 2 µg/mg of lysate * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Protein A purified |
| 缓冲液 | 1X PBS buffer with < 0.1% sodium azide. |
| 抗菌剂 | < 0.1% sodium azide. |
| 浓度 | 2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P07992 Human DNA excision repair protein ERCC-1 |
|---|---|
| 基因名称 | ERCC1 |
| 全名 | excision repair cross-complementation group 1 |
| 背景介绍 | The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. [provided by RefSeq, Oct 2009] |
| 生物功能 | Isoform 1: Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4. [UniProt] |
| 细胞定位 | Nucleus |
| 研究领域 | Cancer antibody; Gene Regulation antibody |
| 预测分子量 | 33 kDa |
克隆号文献
Subcellular distribution of a fluorescence-labeled combi-molecule designed to block epidermal growth factor receptor tyrosine kinase and damage DNA with a green fluorescent species.
WB / Human
The ERCC1/XPF endonuclease is required for completion of homologous recombination at DNA replication forks stalled by inter-strand cross-links.
WB / Human
Development of an unsupervised pixel-based clustering algorithm for compartmentalization of immunohistochemical expression using Automated QUantitative Analysis.
IHC / Human
ERCC1 expression and RAD51B activity correlate with cell cycle response to platinum drug treatment not DNA repair.
WB / Human
Tumors established with cell lines selected for oxaliplatin resistance respond to oxaliplatin if combined with cetuximab.
WB / Human
