ARG51811
anti-ERBB3 / HER3 phospho (Tyr1328) antibody
anti-ERBB3 / HER3 phospho (Tyr1328) antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Controls and Markers antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ERBB3 / HER3 phospho (Tyr1328) |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ERBB3 / HER3 |
| 抗原物种 | Human |
| 抗原 | Peptide sequence around phosphorylation site of tyrosine 1328 (P-D-Y(p)-W-H) derived from Human Her3/ErbB3. |
| 偶联标记 | Un-conjugated |
| 別名 | MDA-BF-1; LCCS2; p180-ErbB3; Proto-oncogene-like protein c-ErbB-3; c-erbB3; p85-sErbB3; Tyrosine kinase-type cell surface receptor HER3; p45-sErbB3; erbB3-S; HER3; c-erbB-3; ErbB-3; EC 2.7.10.1; Receptor tyrosine-protein kinase erbB-3 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| 缓冲液 | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ERBB3 |
| 全名 | erb-b2 receptor tyrosine kinase 3 |
| 背景介绍 | Binds and is activated by neuregulins and NTAK. |
| 生物功能 | Binds and is activated by neuregulins and NTAK. May also be activated by CSPG5. [UniProt] |
| 研究领域 | Cancer antibody; Controls and Markers antibody; Signaling Transduction antibody |
| 预测分子量 | 148 kDa |
| 翻译后修饰 | Autophosphorylated (PubMed:20351256). Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (PubMed:20682778). |
检测图片 (3) Click the Picture to Zoom In
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ARG51811 anti-ERBB3 / HER3 phospho (Tyr1328) antibody WB image
Western blot: Extracts from A431 cells untreated or treated with EGF stained with ARG51811 anti-ERBB3 / HER3 phospho (Tyr1328) antibody.
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ARG51811 anti-ERBB3 / HER3 phospho (Tyr1328) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51811 anti-ERBB3 / HER3 phospho (Tyr1328) antibody (left) or the same antibody preincubated with blocking peptide (right).
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ARG51811 anti-ERBB3 / HER3 phospho (Tyr1328) antibody ICC/IF image
Immunofluorescence: methanol-fixed MCF7 cells stained with ARG51811 anti-ERBB3 / HER3 phospho (Tyr1328) antibody.
