ARG55546
anti-ERBB3 / HER3 antibody
anti-ERBB3 / HER3 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Controls and Markers antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ERBB3 / HER3 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ERBB3 / HER3 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 24-55 (N-terminus) of Human ERBB3. |
| 偶联标记 | Un-conjugated |
| 別名 | MDA-BF-1; LCCS2; p180-ErbB3; Proto-oncogene-like protein c-ErbB-3; c-erbB3; p85-sErbB3; Tyrosine kinase-type cell surface receptor HER3; p45-sErbB3; erbB3-S; HER3; c-erbB-3; ErbB-3; EC 2.7.10.1; Receptor tyrosine-protein kinase erbB-3 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Mouse brain |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P21860 Human Receptor tyrosine-protein kinase erbB-3 Swiss-port # Q61526 Mouse Receptor tyrosine-protein kinase erbB-3 |
|---|---|
| 基因名称 | ERBB3 |
| 全名 | erb-b2 receptor tyrosine kinase 3 |
| 背景介绍 | This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized. [provided by RefSeq, Jul 2008] |
| 生物功能 | Binds and is activated by neuregulins and NTAK. May also be activated by CSPG5. [UniProt] |
| 细胞定位 | Isoform 1: Cell membrane; Single-pass type I membrane protein |
| 研究领域 | Cancer antibody; Controls and Markers antibody; Signaling Transduction antibody |
| 预测分子量 | 148 kDa |
| 翻译后修饰 | Autophosphorylated (PubMed:20351256). Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (PubMed:20682778). |
检测图片 (3) Click the Picture to Zoom In
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ARG55546 anti-ERBB3 / HER3 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast carcinoma stained with ARG55546 anti-ERBB3 / HER3 antibody.
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ARG55546 anti-ERBB3 / HER3 antibody WB image
Western blot: Mouse brain lysate stained with ARG55546 anti-ERBB3 / HER3 antibody.
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ARG55546 anti-ERBB3 / HER3 antibody FACS image
Flow Cytometry: 293 cells stained with ARG55546 anti-ERBB3 / HER3 antibody (bottom histogram) or without primary antibody control (top histogram), followed by incubation with FITC labelled secondary antibody.
