ARG43450
anti-EPAS1 / HIF-2 alpha antibody
anti-EPAS1 / HIF-2 alpha antibody for Flow cytometry,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes EPAS1 / HIF-2 alpha |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | EPAS1 / HIF-2 alpha |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide between aa. 124-150 of Human EPAS1 / HIF-2 alpha. |
| 偶联标记 | Un-conjugated |
| 別名 | Basic-helix-loop-helix-PAS protein MOP2; HIF2A; HIF-1-alpha-like factor; HIF2-alpha; PAS domain-containing protein 2; Endothelial PAS domain-containing protein 1; HIF-2-alpha; MOP2; Hypoxia-inducible factor 2-alpha; bHLHe73; Member of PAS protein 2; ECYT4; HLF; Class E basic helix-loop-helix protein 73; PASD2; EPAS-1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | A549 | ||||||
| 实际分子量 | ~ 96 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q99814 Human Endothelial PAS domain-containing protein 1 |
|---|---|
| 基因名称 | EPAS1 |
| 全名 | endothelial PAS domain protein 1 |
| 背景介绍 | This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009] |
| 生物功能 | Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity). Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD (By similarity). [UniProt] |
| 细胞定位 | Nucleus. Nucleus speckle. Note=Colocalizes with HIF3A in the nucleus and speckles. [UniProt] |
| 预测分子量 | 96 kDa |
| 翻译后修饰 | In normoxia, is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization (By similarity). In normoxia, is hydroxylated on Asn-847 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation. Phosphorylated on multiple sites in the CTAD. The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG43450 anti-EPAS1 / HIF-2 alpha antibody WB image
Western blot: 35 µg of A549 cell lysate stained with ARG43450 anti-EPAS1 / HIF-2 alpha antibody.
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ARG43450 anti-EPAS1 / HIF-2 alpha antibody FACS image
Flow Cytometry: A549 cells stained with ARG43450 anti-EPAS1 / HIF-2 alpha antibody (right histogram) or without primary antibody as control (left histogram), followed by incubation with FITC labelled secondary antibody.
