ARG22202
anti-ENaC gamma antibody
anti-ENaC gamma antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Hamster,Xenopus laevis
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ENaC gamma |
|---|---|
| 反应物种 | Hu, Ms, Rat, Hm, Xenopus laevis |
| 应用 | ICC/IF, IHC-P, WB |
| 特异性 | Detects ~83kDa. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ENaC gamma |
| 抗原物种 | Rat |
| 抗原 | Synthetic peptide around aa. 629-650 (C-terminus) of Rat ENaC Gamma |
| 偶联标记 | Un-conjugated |
| 別名 | ENaCG; Epithelial Na; Gamma-NaCH; Gamma-ENaC; ENaCg; PHA1; SCNEG; BESC3; ENaCgamma; Amiloride-sensitive sodium channel subunit gamma; Nonvoltage-gated sodium channel 1 subunit gamma |
应用说明
| 应用建议 |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.09% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | Scnn1g |
| 全名 | sodium channel, non-voltage-gated 1, gamma subunit |
| 背景介绍 | Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the gamma subunit, and mutations in this gene have been associated with Liddle syndrome. [provided by RefSeq, Apr 2009] |
| 生物功能 | Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception. [UniProt] |
| 细胞定位 | Apical cell membrane |
| 预测分子量 | 74 kDa |
| 翻译后修饰 | Phosphorylated on serine and threonine residues. Aldosterone and insulin increase the basal level of phosphorylation. Ubiquitinated; this targets individual subunits for endocytosis and proteasome-mediated degradation. ENaC cleavage by furin, and subsequently by prostasin (PRSS8), leads to a stepwise increase in the open probability of the channel as a result of release of the alpha and gamma subunit inhibitory tracts, respectively. Interaction of ENaC subunit SCNN1B with BPIFA1 protects ENaC against proteolytic activation. |
检测图片 (3) Click the Picture to Zoom In
-
ARG22202 anti-ENaC gamma antibody IHC image
Immunohistochemistry: Rat kidney tissue stained with ARG22202 anti-ENaC gamma antibody.
-
ARG22202 anti-ENaC gamma antibody WB image
Western blot: Rat kidney tissue lysates stained with ARG22202 anti-ENaC gamma antibody.
-
ARG22202 anti-ENaC gamma antibody WB image
Western blot: Mouse kidney cortex stained with ARG22202 anti-ENaC gamma antibody at 1:1000 dilution. Low-salt diet (lanes 1-4) compared to a high-salt diet (lanes 5-7). 70kDa degradation band observed in low-salt.
