ARG42135
anti-ENC1 antibody
anti-ENC1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
概述
| 产品描述 | Goat Polyclonal antibody recognizes ENC1 |
|---|---|
| 反应物种 | Hu, Rat |
| 预测物种 | Ms, Cow, Dog, Pig |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ENC1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the internal region of Human ENC1. (TSVSHDKLPKVQC) (NP_003624.1; NP_001243505.1) |
| 偶联标记 | Un-conjugated |
| 別名 | KLHL37; KLHL35; Kelch-like protein 37; TP53I10; PIG10; NRPB; Nuclear matrix protein NRP/B; CCL28; ENC-1; Ectoderm-neural cortex protein 1; p53-induced gene 10 protein |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||
| 阳性对照 | Rat ovary | ||||||
| 实际分子量 | ~ 70 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ENC1 |
| 全名 | ectodermal-neural cortex 1 (with BTB domain) |
| 背景介绍 | This gene encodes a member of the kelch-related family of actin-binding proteins. The encoded protein plays a role in the oxidative stress response as a regulator of the transcription factor Nrf2, and expression of this gene may play a role in malignant transformation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012] |
| 生物功能 | Actin-binding protein involved in the regulation of neuronal process formation and in differentiation of neural crest cells. Down-regulates transcription factor NF2L2/NRF2 by decreasing the rate of protein synthesis and not via a ubiquitin-mediated proteasomal degradation mechanism. [UniProt] |
| 细胞定位 | Nucleus matrix. Cytoplasm. Cytoplasm, cytoskeleton. [UniProt] |
| 预测分子量 | 66 kDa |
| 翻译后修饰 | Ubiquitinated by E3 ubiquitin ligase complex formed by CUL3 and RBX1 and probably targeted for proteasome-independent degradation. Quinone-induced oxidative stress increases its ubiquitination. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
-
ARG42135 anti-ENC1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human adrenal gland tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG42135 anti-ENC1 antibody at 5 µg/ml dilution followed by AP-staining.
-
ARG42135 anti-ENC1 antibody WB image
Western blot: 35 µg of Rat ovary lysate (in RIPA buffer) stained with ARG42135 anti-ENC1 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
