ARG42130

anti-ENC1 antibody

anti-ENC1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat

概述

产品描述 Chicken Polyclonal antibody recognizes ENC1
反应物种 Ms, Rat
应用 IHC-P, WB
宿主 Chicken
克隆 Polyclonal
同位型 IgY
靶点名称 ENC1
抗原物种 Human
抗原 A 13 amino acid synthetic peptide within aa. 230 - 280 of Human ENC1.
偶联标记 Un-conjugated
別名 KLHL37; KLHL35; Kelch-like protein 37; TP53I10; PIG10; NRPB; Nuclear matrix protein NRP/B; CCL28; ENC-1; Ectoderm-neural cortex protein 1; p53-induced gene 10 protein

应用说明

应用建议
应用 推荐稀释比
IHC-P5 - 20 µg/ml
WB1 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 ~ 51 kDa

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS and 0.02% Sodium azide.
抗菌剂 0.02% Sodium azide
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 13803 Mouse ENC1

Swiss-port # O35709 Mouse Ectoderm-neural cortex protein 1

基因名称 ENC1
全名 ectodermal-neural cortex 1 (with BTB domain)
背景介绍 This gene encodes a member of the kelch-related family of actin-binding proteins. The encoded protein plays a role in the oxidative stress response as a regulator of the transcription factor Nrf2, and expression of this gene may play a role in malignant transformation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]
生物功能 Actin-binding protein involved in the regulation of neuronal process formation and in differentiation of neural crest cells. Down-regulates transcription factor NF2L2/NRF2 by decreasing the rate of protein synthesis and not via a ubiquitin-mediated proteasomal degradation mechanism. [UniProt]
细胞定位 Nucleus matrix. Cytoplasm. Cytoplasm, cytoskeleton. [UniProt]
预测分子量 66 kDa
翻译后修饰 Ubiquitinated by E3 ubiquitin ligase complex formed by CUL3 and RBX1 and probably targeted for proteasome-independent degradation. Quinone-induced oxidative stress increases its ubiquitination. [UniProt]

检测图片 (3) Click the Picture to Zoom In

  • ARG42130 anti-ENC1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat kidney tissue stained with ARG42130 anti-ENC1 antibody at 5 µg/ml dilution.

  • ARG42130 anti-ENC1 antibody WB image

    Western blot: Mouse kidney muscle lysate stained with ARG42130 anti-ENC1 antibody at 1 µg/ml dilution, in the absence (left) and the presence (right) of blocking peptide.

  • ARG42130 anti-ENC1 antibody IHC image

    Immunohistochemistry: Rat kidney tissue stained with ARG42130 anti-ENC1 antibody at 20 µg/ml dilution.