ARG53010

anti-EMR1 F4/80 antibody [SP115]

anti-EMR1 F4/80 antibody [SP115] for IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Human,Mouse

Controls and Markers antibody; Immune System antibody
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anti-EMR1 F4/80 antibody [SP115]

Rabbit Monoclonal antibody [SP115] recognizes EMR1 F4/80

ARG53010 Arigo biolaboratories
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anti-EMR1 F4/80 antibody [SP115] is rated 5.0 out of 5 by 1.

anti-EMR1 F4/80 antibody [SP115]

Overview

Product Description

Rabbit Monoclonal antibody [SP115] recognizes EMR1 F4/80

Tested Reactivity Hu, Ms
Predict Reactivity Rat
Tested Application IHC-Fr, IHC-P, WB
Host Rabbit
Clonality Monoclonal
Clone SP115
Isotype IgG
Target Name EMR1 F4/80
Antigen Species Mouse
Immunogen Synthetic peptide around the N-terminus of Mouse EMR1 F4/80.
Conjugation Un-conjugated
Alternate Names EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; EMR1; Adhesion G protein-coupled receptor E1; EGF-like module receptor 1; TM7LN3

Application Instructions

Application Suggestion
Application Dilution
IHC-Fr1:100 - 1:200
IHC-P1:100
WB1:500 - 1:2000
Application Note IHC-P: Antigen Retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.
Incubation Time: 10 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human Lung, Fresh Cut Mouse Liver
Calculated MW 160 kDa

Properties

Form Liquid
Purification Purified by protein A/G
Buffer PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 13733 Mouse ADGRE1

GeneID: 2015 Human ADGRE1

Swiss-port # Q14246 Human Adhesion G protein-coupled receptor E1

Swiss-port # Q61549 Mouse Adhesion G protein-coupled receptor E1

Background Mouse EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1) or cell surface glycoprotein F4/80 is a cell surface glycoprotein and a member of the EGF TM7 family of proteins. It shares 68% overall amino acid identity with human EGF-module-containing-mucin-like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ regulatory T cells.
Cellular Localization Membrane
Research Area Controls and Markers antibody; Immune System antibody

Images (5) Click the Picture to Zoom In

  • ARG53010 anti-EMR1 F4/80 antibody [SP115] WB image

    Western blot: 30 µg of Mouse lung lysate stained with ARG53010 anti-EMR1 F4/80 antibody [SP115] at 1:1000 dilution.

  • ARG53010 anti-EMR1 F4/80 antibody [SP115] IHC-P image

    Immunohistochemistry: Human colon stained with ARG53010 anti-EMR1 F4/80 antibody [SP115].

  • ARG53010 anti-EMR1 F4/80 antibody [SP115] IHC-P image

    Immunohistochemistry: Human Esophagus stained with ARG53010 anti-EMR1 F4/80 antibody [SP115].

  • ARG53010 anti-EMR1 F4/80 antibody [SP115] IHC-P image

    Immunohistochemistry: Mouse Liver stained with ARG53010 anti-EMR1 F4/80 antibody [SP115].

  • ARG53010 anti-EMR1 F4/80 antibody [SP115] IHC-P image

    Immunohistochemistry: Mouse Liver stained with ARG53010 anti-EMR1 F4/80 antibody [SP115].

Customer's Feedback

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Review for anti-EMR1 F4/80 antibody [SP115]

Application:WB

Sample:Mouse lung

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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Clone References

A small peptide with potential ability to promote wound healing.

IHC / Mouse

Tang J et al.
PLoS One.,  (2014)

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