ARG63882
anti-EMI2 antibody
anti-EMI2 antibody for Western blot and Mouse
Cell Biology and Cellular Response antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes EMI2 |
|---|---|
| 反应物种 | Ms |
| 预测物种 | Hu, Rat, Cow, Dog, Pig |
| 应用 | WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | EMI2 |
| 抗原物种 | Human |
| 抗原 | C-PGSAQSKRNLKRL |
| 偶联标记 | Un-conjugated |
| 別名 | ERP1; FBX43; EMI2; F-box only protein 43; Endogenous meiotic inhibitor 2 |
应用说明
| 应用建议 |
|
||||
|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 背景介绍 | Members of the F-box protein family, such as FBXO43, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008] |
|---|---|
| 研究领域 | Cell Biology and Cellular Response antibody |
| 预测分子量 | 78 kDa |
| 翻译后修饰 | Phosphorylated on Ser-76, Thr-234 and Ser-334 in response to calcium, which is a prerequisite for ubiquitination and proteasomal degradation. Ubiquitinated in response to calcium, which promotes proteasomal degradation. |
检测图片 (1) Click the Picture to Zoom In
-
ARG63882 anti-EMI2 antibody WB image
Western blot: 35 µg of Mouse ovary lysate (in RIPA buffer) stained with ARG63882 anti-EMI2 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
