ARG63176

anti-ELMO1 antibody

anti-ELMO1 antibody for Immunoprecipitation,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody
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概述

产品描述 Goat Polyclonal antibody recognizes ELMO1
反应物种 Hu, Ms, Rat
预测物种 Cow, Dog, Zfsh
应用 IP, WB
特异性 This antibody is expected to recognise both human isoforms of this protein. NP_569709.1 and NP_001034548.1 represent the same isoform.
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 ELMO1
抗原物种 Human
抗原 PKEPSNYDFVYDCN
偶联标记 Un-conjugated
別名 Protein ced-12 homolog; ELMO-1; CED12; CED-12; Engulfment and cell motility protein 1

应用说明

应用建议
应用 推荐稀释比
IPAssay - dependent
WB0.1 - 0.3 µg/ml
应用说明 WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified from goat serum by antigen affinity chromatography.
缓冲液 Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂 0.02% Sodium azide
稳定剂 0.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 140580 Mouse ELMO1

GeneID: 9844 Human ELMO1

Swiss-port # Q8BPU7 Mouse Engulfment and cell motility protein 1

Swiss-port # Q92556 Human Engulfment and cell motility protein 1

背景介绍 This gene encodes a member of the engulfment and cell motility protein family. These proteins interact with dedicator of cytokinesis proteins to promote phagocytosis and cell migration. Increased expression of this gene and dedicator of cytokinesis 1 may promote glioma cell invasion, and single nucleotide polymorphisms in this gene may be associated with diabetic nephropathy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2011]
研究领域 Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody
预测分子量 84 kDa
翻译后修饰 Phosphorylated by HCK.

检测图片 (2) Click the Picture to Zoom In

  • ARG63176 anti-ELMO1 antibody WB image

    Western blot: 35 µg of Human frontal cortex (A), Mouse brain (B) and Rat brain (C) lysates (in RIPA buffer) stained with ARG63176 anti-ELMO1 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.

  • ARG63176 anti-ELMO1 antibody WB image

    Western blot: 35 µg of Jurkat cell lysate (in RIPA buffer) stained with ARG63176 anti-ELMO1 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.

客户反馈

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anti-ELMO1 antibody

Application:WB

Sample:Rat brain

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Good

anti-ELMO1 antibody

Application:WB

Sample:Mouse brain

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

anti-ELMO1 antibody

Application:WB

Sample:Jurkat

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC