ARG52272
anti-ELK1 phospho (Ser383) antibody
anti-ELK1 phospho (Ser383) antibody for Western blot and Rat
Cancer antibody; Gene Regulation antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ELK1 phospho (Ser383) |
|---|---|
| 反应物种 | Rat |
| 预测物种 | Hu, Ms, Dog, NHuPrm, Zfsh |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ELK1 |
| 抗原物种 | Human |
| 抗原 | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser383 conjugated to KLH |
| 偶联标记 | Un-conjugated |
| 別名 | ETS domain-containing protein Elk-1 |
应用说明
| 应用建议 |
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| 应用说明 | Specific for ~46k Elk-1 phosphorylated at Ser383. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | ELK1 |
|---|---|
| 全名 | ELK1, member of ETS oncogene family |
| 背景介绍 | Elk-1 is a transcription factor involved in mediating gene transcription in response to growth factors (Hill and Treisman, 1995). Elk-1 is thought to be phosphorylated by MAP kinase at Ser383 and phosphorylation at this site is essential for the transcriptional activity of Elk-1 (Li et al., 2003). Phosphorylation of Elk-1 has also been implicated in synaptic plasticity in the adult hippocampus (Thiels et al., 2002). |
| 研究领域 | Cancer antibody; Gene Regulation antibody; Signaling Transduction antibody |
| 预测分子量 | 45 kDa |
| 翻译后修饰 | Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention. On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Also phosphorylated on Ser-383 by MAPK8 and/or MAKP9. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. Phosphorylated and activated by CAMK4, MAPK11, MAPK12 and MAPK14. Upon bFGF stimulus, phosphorylated by PAK1 (By similarity). |
检测图片 (1) Click the Picture to Zoom In
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ARG52272 anti-ELK1 phospho (Ser383) antibody WB image
Western blot: non-phospho- and phospho-recombinant Elk-1 showing specific immunolabeling of the ~46 Elk-1 protein phosphorylated at Ser 383 stained with ARG52272 anti-ELK1 phospho (Ser383) antibody.
