ARG63462
anti-ELF1 antibody
anti-ELF1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Gene Regulation antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes ELF1 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | IHC-P, WB |
| 特异性 | This antibody is expected to recognise both reported isoforms. |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ELF1 |
| 抗原物种 | Human |
| 抗原 | C-AMKQNELLEPNSF |
| 偶联标记 | Un-conjugated |
| 別名 | E74-like factor 1; ETS-related transcription factor Elf-1 |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P32519 Human ETS-related transcription factor Elf-1 Swiss-port # Q60775 Mouse ETS-related transcription factor Elf-1 |
|---|---|
| 背景介绍 | This gene encodes an E26 transformation-specific related transcription factor. The encoded protein is primarily expressed in lymphoid cells and acts as both an enhancer and a repressor to regulate transcription of various genes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2009] |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 67 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG63462 anti-ELF1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human thymus tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63462 anti-ELF1 antibody at 2.5 µg/ml dilution followed by AP-staining.
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ARG63462 anti-ELF1 antibody WB image
Western blot: 35 µg of Human lymph node (A), Human tonsil (B) and Mouse thymus (C) lysates (in RIPA buffer) stained with ARG63462 anti-ELF1 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
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ARG63462 anti-ELF1 antibody WB image
Western blot: 35 µg of Jurkat cell lysate (in RIPA buffer) stained with ARG63462 anti-ELF1 antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
