ARG55880
anti-EHMT2 / G9A antibody
anti-EHMT2 / G9A antibody for Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes EHMT2 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | EHMT2 / G9A |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 361-395 (Center) of Human EHMT2. |
| 偶联标记 | Un-conjugated |
| 別名 | H3-K9-HMTase 3; GAT8; KMT1C; G9A; Euchromatic histone-lysine N-methyltransferase 2; Histone H3-K9 methyltransferase 3; Protein G9a; NG36; Lysine N-methyltransferase 1C; C6orf30; HLA-B-associated transcript 8; EC 2.1.1.43; Histone-lysine N-methyltransferase EHMT2; EC 2.1.1.-; BAT8 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | A431 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q96KQ7 Human Histone-lysine N-methyltransferase EHMT2 Swiss-port # Q9Z148 Mouse Histone-lysine N-methyltransferase EHMT2 |
|---|---|
| 基因名称 | EHMT2 |
| 全名 | euchromatic histone-lysine N-methyltransferase 2 |
| 背景介绍 | This gene is located in a gene cluster within the human major histocompatibility complex class III region on chromosome 6. This gene was thought to be two different genes, NG36 and G9a, adjacent to each other but recent publications show that there is only a single gene at this locus. The encoded protein is a histone methyltransferase, which may be involved in repression of transcription. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| 生物功能 | Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself. [UniProt] |
| 细胞定位 | Nucleus. Chromosome. Note=Associates with euchromatic regions. Does not associate with heterochromatin |
| 预测分子量 | 132 kDa |
| 翻译后修饰 | Methylated at Lys-185; automethylated. |
检测图片 (1) Click the Picture to Zoom In
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ARG55880 anti-EHMT2 / G9A antibody WB image
Western blot: 20 µg of A431 cell lysate stained with ARG55880 anti-EHMT2 / G9A antibody at 1:1000 dilution.
