ARG58653
anti-EHHADH antibody
anti-EHHADH antibody for Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes EHHADH |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | EHHADH |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 444-723 of Human EHHADH (NP_001957.2). |
| 偶联标记 | Un-conjugated |
| 別名 | LBP; ECHD; LBFP; L-PBE; PBE; PBFE; EC 1.1.1.35; EC 4.2.1.17; EC 5.3.3.8; FRTS3; Peroxisomal bifunctional enzyme |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | SW480 | ||||
| 实际分子量 | 79 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | EHHADH |
| 全名 | enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase |
| 背景介绍 | The protein encoded by this gene is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in this gene are a cause of peroxisomal disorders such as Zellweger syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009] |
| 细胞定位 | Peroxisome. [UniProt] |
| 预测分子量 | 79 kDa |
| 翻译后修饰 | Acetylated, leading to enhanced enzyme activity. Acetylation is enhanced by up to 80% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with highest increase on Lys-346. Acetylation and enzyme activity increased by about 1.5% on addition of fatty acids. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG58653 anti-EHHADH antibody WB image
Western blot: 25 µg of SW480 cell lysate stained with ARG58653 anti-EHHADH antibody at 1:1000 dilution.
