ARG58604
anti-EHHADH antibody
anti-EHHADH antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes EHHADH |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | EHHADH |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide between aa. 662-690 of Human EHHADH. |
| 偶联标记 | Un-conjugated |
| 別名 | LBP; ECHD; LBFP; L-PBE; PBE; PBFE; EC 1.1.1.35; EC 4.2.1.17; EC 5.3.3.8; FRTS3; Peroxisomal bifunctional enzyme |
应用说明
| 应用建议 |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Rat liver |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | EHHADH |
| 全名 | enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase |
| 背景介绍 | The protein encoded by this gene is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in this gene are a cause of peroxisomal disorders such as Zellweger syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009] |
| 细胞定位 | Peroxisome. [UniProt] |
| 预测分子量 | 79 kDa |
| 翻译后修饰 | Acetylated, leading to enhanced enzyme activity. Acetylation is enhanced by up to 80% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with highest increase on Lys-346. Acetylation and enzyme activity increased by about 1.5% on addition of fatty acids. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
-
ARG58604 anti-EHHADH antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human hepatocarcinoma tissue stained with ARG58604 anti-EHHADH antibody.
-
ARG58604 anti-EHHADH antibody WB image
Western blot: 35 µg of Rat liver lysate stained with ARG58604 anti-EHHADH antibody at 1:1000 dilution.
-
ARG58604 anti-EHHADH antibody FACS image
Flow Cytometry: HepG2 cells stained with ARG58604 anti-EHHADH antibody (bottom histogram) or without primary antibody as control (top histogram), followed by incubation with FITC labelled secondary antibody.
