ARG45557

anti-EHD3 antibody

anti-EHD3 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Monkey

概述

产品描述 Rabbit Polyclonal antibody recognizes EHD3
反应物种 Hu, Ms, Rat, Mk
应用 FACS, ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 EHD3
抗原物种 Human
抗原 Synthetic peptide corresponding to C-terminal region of human EHD3.
偶联标记 Un-conjugated
別名 EHD3; EH Domain Containing 3; PAST3; EH Domain-Containing Protein 3; PAST Homolog 3; EHD2

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
IHC-P2-5 μg/ml
WB0.1-0.25 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 70 kDa

属性

形式 Liquid
纯化 Affinity purified
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 192249 Rat EHD3

GeneID: 30845 Human EHD3

GeneID: 57440 Mouse EHD3

基因名称 EHD3
全名 EH Domain Containing 3
背景介绍 Predicted to enable nucleic acid binding activity. Involved in several processes, including cytosolic transport; endocytic recycling; and protein homooligomerization. Acts upstream of or within protein localization to plasma membrane and regulation of cardiac muscle cell membrane potential. Located in ciliary pocket membrane and recycling endosome membrane. [provided by Alliance of Genome Resources, Feb 2025]
生物功能 TP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. In vitro causes tubulation of endocytic membranes. Binding to phosphatidic acid induces its membrane tubulation activity (By similarity). Plays a role in endocytic transport. Involved in early endosome to recycling endosome compartment (ERC), retrograde early endosome to Golgi, and endosome to plasma membrane (rapid recycling) protein transport. Involved in the regulation of Golgi maintenance and morphology. [UniProt]
细胞定位 Cell membrane; Cell projection; Cilium; Endosome; Membrane. [UniProt]
预测分子量 61 kDa
翻译后修饰 Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation. [UniProt]

检测图片 (6) Click the Picture to Zoom In

  • ARG45557 anti-EHD3 antibody IHC-P image

    Immunohistochemistry: Human ovarian serous adenocarcinoma stained with ARG45557 anti-EHD3 antibody at 2 μg/ml dilution.

  • ARG45557 anti-EHD3 antibody ICC/IF image

    Immunofluorescence: MCF-7 stained with ARG45557 anti-EHD3 antibody at 5 μg/ml dilution.

  • ARG45557 anti-EHD3 antibody WB image

    Western blot: A549 stained with ARG45557 anti-EHD3 antibody at 0.5 μg/ml dilution.

  • ARG45557 anti-EHD3 antibody FACS image

    Flow Cytometry: MCF-7 stained with ARG45557 anti-EHD3 antibody at 1 µg/10^6 cells dilution.

  • ARG45557 anti-EHD3 antibody IHC-P image

    Immunohistochemistry: Rat brain stained with ARG45557 anti-EHD3 antibody at 2 μg/ml dilution.

  • ARG45557 anti-EHD3 antibody WB image

    Western blot: monkey heart stained with ARG45557 anti-EHD3 antibody at 0.5 μg/ml dilution.