ARG64290

anti-EGR2 antibody

anti-EGR2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Gene Regulation antibody; Neuroscience antibody

概述

产品描述 Goat Polyclonal antibody recognizes EGR2
反应物种 Hu
预测物种 Ms, Rat, Dog
应用 IHC-P, WB
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 EGR2
抗原物种 Human
抗原 HGTAGPDRKPFPC
偶联标记 Un-conjugated
別名 KROX20; Zinc finger protein Krox-20; EC 6.3.2.-; CMT1D; Early growth response protein 2; EGR-2; AT591; E3 SUMO-protein ligase EGR2; CMT4E

应用说明

应用建议
应用 推荐稀释比
IHC-PAssay - dependent
WB2 - 6 µg/ml
应用说明 WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified from goat serum by antigen affinity chromatography.
缓冲液 Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂 0.02% Sodium azide
稳定剂 0.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 1959 Human EGR2

Swiss-port # P11161 Human E3 SUMO-protein ligase EGR2

背景介绍 The protein encoded by this gene is a transcription factor with three tandem C2H2-type zinc fingers. Defects in this gene are associated with Charcot-Marie-Tooth disease type 1D (CMT1D), Charcot-Marie-Tooth disease type 4E (CMT4E), and with Dejerine-Sottas syndrome (DSS). Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]
研究领域 Gene Regulation antibody; Neuroscience antibody
预测分子量 50 kDa
翻译后修饰 Ubiquitinated by WWP2 leading to proteasomal degradation.

检测图片 (3) Click the Picture to Zoom In

  • ARG64290 anti-EGR2 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human small intestine tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64290 anti-EGR2 antibody at 3.8 µg/ml dilution followed by AP-staining.

  • ARG64290 anti-EGR2 antibody IHC-P image

    Immunohistochemistry: paraffin embedded Human Colon. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64290 anti-EGR2 antibody at 3.8 µg/ml dilution followed by AP-staining.

  • ARG64290 anti-EGR2 antibody WB image

    Western blot: 35 µg of HepG2 cell lysate (in RIPA buffer) stained with ARG64290 anti-EGR2 antibody at 2 µg/ml dilution and incubated at RT for 1 hour.