ARG10200
anti-EGF antibody [S-146] (HRP)
anti-EGF antibody [S-146] (HRP) for ELISA and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody
概述
| 产品描述 | HRP-conjugated Mouse Monoclonal antibody [S-146] recognizes Human EGF |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | Does not cross react with other human cytokines tested such as IL-1β, IL-8, MCAF and TGF-β. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | S-146 |
| 同位型 | IgG1, kappa |
| 靶点名称 | EGF |
| 抗原物种 | Human |
| 抗原 | Purified recombinant human EGF, 6kDa, 53 amino acid residues |
| 偶联标记 | HRP |
| 別名 | Urogastrone; Pro-epidermal growth factor; URG; HOMG4; EGF |
应用说明
| 应用说明 | ELISA: This HRP conjugated antibody can be used in sandwich ELISA for EGF detection. The matched antibody (capture) is clone S-134 (Anogen Cat No.: ARG10199). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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属性
| 形式 | Liquid |
|---|---|
| 纯化 | Protein G affinity purified |
| 缓冲液 | 0.01M PBS (pH 7.2) and 50% Glycerol |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Keep the antibody in the dark and keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | EGF |
| 全名 | epidermal growth factor |
| 背景介绍 | Epidermal growth factor (EGF), along with its receptor EGFR, plays an important role in cell proliferation, differentiation and survival. The binding of EGF to EGFR triggers the intrinsic tyrosine kinase activity of EGFR, activates signalling molecules and stimulates the synthesis of proteins and DNAs. EGF is crucial for maintaining mucosal lining in gastrointestinal duct and for healing of oral and gastroesophageal ulcers. EGF signalling is needed for cancer growth. Monoclonal antibodies that block EGFR have been applied for lung cancer and colon inhibition. |
| 生物功能 | EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941). [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30073 EGF ELISA Antibody Duo Related products: EGF antibodies; EGF ELISA Kits; EGF Duos / Panels; Anti-Mouse IgG secondary antibodies; |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody |
| 预测分子量 | 134 kDa |
| 翻译后修饰 | O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor). |
