ARG56714
anti-EGF antibody
anti-EGF antibody for ELISA,IHC-Frozen sections,Neutralizing,Western blot and Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes EGF |
|---|---|
| 反应物种 | Rat |
| 应用 | ELISA, IHC-Fr, Neut, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | EGF |
| 抗原物种 | Rat |
| 抗原 | E.coli derived Recombinant Rat EGF. (MNSNTGCPPS YDGYCLNGGV CMYVESVDRY VCNCVIGYIG ERCQHRDLRW WKLR) |
| 偶联标记 | Un-conjugated |
| 別名 | Urogastrone; Pro-epidermal growth factor; URG; HOMG4; EGF |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.2) |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | Egf |
| 全名 | epidermal growth factor |
| 背景介绍 | This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010] |
| 生物功能 | EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro. [UniProt] |
| 预测分子量 | 134 kDa |
| 翻译后修饰 | O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor). |
检测图片 (3) Click the Picture to Zoom In
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ARG56714 anti-EGF antibody WB image
Western blot: 250 - 0.24 ng of Rat EGF stained with ARG56714 anti-EGF antibody, under reducing conditions.
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ARG56714 anti-EGF antibody WB image
Western blot: 250 - 0.24 ng of Rat EGF stained with ARG56714 anti-EGF antibody, under non-reducing conditions.
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ARG56714 anti-EGF antibody standard curve image
Sandwich ELISA: ARG56714 anti-EGF antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56823 anti-EGF antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.
