ARG42138
anti-ECSM2 antibody
anti-ECSM2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Goat Polyclonal antibody recognizes ECSM2 |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ECSM2 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the C-terminus of Human ECSM2. (C-NNGKQSLSAEKVL) (NP_001071161.1) |
| 偶联标记 | Un-conjugated |
| 別名 | Apoptosis regulator through modulating IAP expression; ARIA; Endothelial cell-specific molecule 2; Endothelial cell-specific chemotaxis regulator; ECSM2 |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||
| 阳性对照 | Human lung and Human umbilical cord. | ||||||
| 实际分子量 | ~ 50 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q19T08 Human Endothelial cell-specific chemotaxis regulator |
|---|---|
| 基因名称 | ECSCR |
| 全名 | endothelial cell surface expressed chemotaxis and apoptosis regulator |
| 背景介绍 | The protein encoded by this gene is primarily found in endothelial cells and blood vessels, where it is involved in cell shape changes and EGF-induced cell migration. It can enhance the activation of vascular endothelial growth factor receptor-2/kinase insert domain receptor and also promote the proteolysis of internalized kinase insert domain receptor. This gene may play a role in angiogenesis-related diseases. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014] |
| 生物功能 | Regulates endothelial chemotaxis and tube formation. Has a role in angiogenesis and apoptosis via modulation of the actin cytoskeleton and facilitation of proteasomal degradation of the apoptosis inhibitors BIRC3/IAP1 and BIRC2/IAP2. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein. Cytoplasm. [UniProt] |
| 预测分子量 | 21 kDa |
| 翻译后修饰 | May be heavily O-glycosylated. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
-
ARG42138 anti-ECSM2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human vessel tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG42138 anti-ECSM2 antibody at 5 µg/ml dilution followed by AP-staining.
-
ARG42138 anti-ECSM2 antibody WB image
Western blot: 35 µg of Human lung and Human umbilical cord lysates (in RIPA buffer) stained with ARG42138 anti-ECSM2 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
