ARG40163
anti-EAAT1 antibody
anti-EAAT1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes EAAT1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | EAAT1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide derived from Human EAAT1. |
| 偶联标记 | Un-conjugated |
| 別名 | EAAT1; Sodium-dependent glutamate/aspartate transporter 1; GLAST-1; Excitatory amino acid transporter 1; GLAST1; Solute carrier family 1 member 3; GLAST; EA6 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | Mouse brain |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SLC1A3 |
| 全名 | solute carrier family 1 (glial high affinity glutamate transporter), member 3 |
| 背景介绍 | This gene encodes a member of a member of a high affinity glutamate transporter family. This gene functions in the termination of excitatory neurotransmission in central nervous system. Mutations are associated with episodic ataxia, Type 6. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2014] |
| 生物功能 | Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. [UniProt] |
| 细胞定位 | Cell membrane; Multi-pass membrane protein. [UniProt] |
| 预测分子量 | 60 kDa |
| 翻译后修饰 | Glycosylated. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG40163 anti-EAAT1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain tissue. Antigen Retrieval: Heat mediation was performed in Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then stained with ARG40163 anti-EAAT1 antibody at 1:50 dilution, for 1 hour at room temperature.
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ARG40163 anti-EAAT1 antibody WB image
Western blot: Mouse brain lysate stained with ARG40163 anti-EAAT1 antibody.
