ARG67031
anti-E2F4 antibody [SQab30318]
anti-E2F4 antibody [SQab30318] for IHC-Formalin-fixed paraffin-embedded sections and Human
概述
| 产品描述 | Recombinant rabbit Monoclonal antibody [SQab30318] recognizes E2F4 |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SQab30318 |
| 同位型 | IgG |
| 靶点名称 | E2F4 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide of Human E2F4. |
| 偶联标记 | Un-conjugated |
| Protein Full name | Transcription factor E2F4 |
| 別名 | E2F4, E2F Transcription Factor 4, E2F-4, E2F Transcription Factor 4, P107/P130-Binding, Transcription Factor E2F4, P107/P130-Binding Protein |
应用说明
| 应用建议 |
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| 应用说明 | The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | Human breast cancer |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05%BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | E2F4 |
| 全名 | E2F Transcription Factor 4 |
| 背景介绍 | The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer. [provided by RefSeq, Jul 2008] |
| 生物功能 | Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis. [Uniprot] |
| 细胞定位 | Nucleus |
| 预测分子量 | 44 kDa |
| 翻译后修饰 | Differentially phosphorylated in vivo. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG67031 anti-E2F4 antibody [SQab30318] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded human breast cancer stained with ARG67031 anti-E2F4 antibody [SQab30318].
