ARG58516
anti-E2F2 antibody
anti-E2F2 antibody for Western blot and Human,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes E2F2 |
|---|---|
| 反应物种 | Hu, Rat |
| 预测物种 | Rb |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | E2F2 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to a sequence at the C-terminus of Human E2F2(422-427aa ISDLFDSYDLGDLLIN), identical to the related Mouse sequence. |
| 偶联标记 | Un-conjugated |
| 別名 | Transcription factor E2F2; E2F-2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Thimerosal and 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | E2F2 |
| 全名 | E2F transcription factor 2 |
| 背景介绍 | The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46% amino acid identity to E2F1. [provided by RefSeq, Jul 2008] |
| 生物功能 | Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 48 kDa |
| 翻译后修饰 | Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG58516 anti-E2F2 antibody WB image
Western blot: 50 µg of Rat lung, 50 µg of Rat heart, 50 µg of Rat brain, 50 µg of Rat kidney, 40 µg of HeLa, 40 µg of COLO320, 40 µg of A549, 40 µg of MCF-7 and 40 µg of SMMC-7721 lysates stained with ARG58516 anti-E2F2 antibody at 0.5 µg/ml dilution.
