ARG62474
anti-E2F1 antibody [KH20]
anti-E2F1 antibody [KH20] for Gel supershift assays,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Xenopus laevis
Cancer antibody; Gene Regulation antibody
概述
| 产品描述 | Mouse Monoclonal antibody [KH20] recognizes E2F1 |
|---|---|
| 反应物种 | Hu, Ms, Rat, Xenopus laevis |
| 应用 | GSA, IHC-P, IP, WB |
| 特异性 | The epitope recognized by the antibody resides in the N-terminal region of human E2F1 (amino acids 1-89). |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | KH20 |
| 同位型 | IgG2a |
| 靶点名称 | E2F1 |
| 抗原物种 | Human |
| 抗原 | Recombinant human E2F1 |
| 偶联标记 | Un-conjugated |
| 別名 | RBAP1; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3; RBBP3; pRB-binding protein E2F-1; RBBP-3; E2F-1; Transcription factor E2F1; RBP3; PBR3; RBAP-1 |
应用说明
| 应用说明 | WB: 1-2 ug/ml * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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属性
| 形式 | Liquid |
|---|---|
| 缓冲液 | 0.01 M PBS (pH 7.4), 1% BSA and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 稳定剂 | 1% BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | E2F1 |
| 全名 | E2F transcription factor 1 |
| 背景介绍 | The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq, Jul 2008] |
| 生物功能 | Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:20176812). [UniProt] |
| 研究领域 | Cancer antibody; Gene Regulation antibody |
| 预测分子量 | 47 kDa |
| 翻译后修饰 | Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. Phosphorylation at Ser-364 by CHEK2 stabilizes E2F1 upon DNA damage and regulates its effect on transcription and apoptosis. Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein RB1. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B. |
