ARG63481
anti-E2-230K / UBE2O antibody
anti-E2-230K / UBE2O antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Cell Biology and Cellular Response antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes E2-230K / UBE2O |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow, Dog, Pig |
| 应用 | IHC-P |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | E2-230K / UBE2O |
| 抗原物种 | Human |
| 抗原 | ALLEAGMPECTEDK |
| 偶联标记 | Un-conjugated |
| 別名 | E2-230K; (E3-independent) E2 ubiquitin-conjugating enzyme; E2/E3 hybrid ubiquitin-protein ligase UBE2O (EC 6.3.2.-; EC 6.3.2.19; Ubiquitin carrier protein O; Ubiquitin-conjugating enzyme E2 O; Ubiquitin-conjugating enzyme E2 of 230 kDa; Ubiquitin-conjugating enzyme E2-230K; Ubiquitin-protein ligase O |
应用说明
| 应用建议 |
|
||||
|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9C0C9 Human (E3-independent) E2 ubiquitin-conjugating enzyme |
|---|---|
| 基因名称 | UBE2O |
| 全名 | ubiquitin-conjugating enzyme E2O |
| 生物功能 | E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins (PubMed:23455153, PubMed:24703950). Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity (PubMed:23381138). Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis (PubMed:23455153). Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location (PubMed:24703950). Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASH1, leading to promote endosomal F-actin assembly (PubMed:23452853). [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody |
| 预测分子量 | 141 kDa |
| 翻译后修饰 | Phosphorylated. Phosphorylation affects subcellular location. Ubiquitinated: autoubiquitinates, possibly affecting its subcellular location. |
检测图片 (1) Click the Picture to Zoom In
-
ARG63481 anti-E2-230K / UBE2O antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Heart. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63481 anti-E2-230K / UBE2O antibody at 4 µg/ml dilution followed by HRP-staining.
