ARG59633
anti-E-Ras antibody
anti-E-Ras antibody for Flow cytometry,Western blot and Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes E-Ras |
|---|---|
| 反应物种 | Ms |
| 应用 | FACS, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | E-Ras |
| 抗原物种 | Mouse |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 19-52 of Mouse E-Ras. |
| 偶联标记 | Un-conjugated |
| 別名 | E-Ras; Embryonic stem cell-expressed Ras; HRAS2; GTPase ERas; HRASP |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | F9 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ERAS |
| 全名 | ES cell expressed Ras |
| 背景介绍 | This gene encodes a constitutively active member of the small GTPase Ras protein family. The encoded protein activates the phosphatidylinositol 3-kinase signal transduction pathway in undifferentiated stem cells, but is not expressed in differentiated cells. This gene may be involved in cancer and chemotherapy resistance. [provided by RefSeq, Dec 2012] |
| 生物功能 | Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the tumor-like growth properties of embryonic stem cells (By similarity). [UniProt] |
| 细胞定位 | Cell membrane; Lipid-anchor; Cytoplasmic side. [UniProt] |
| 预测分子量 | 25 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG59633 anti-E-Ras antibody WB image
Western blot: 20 µg of F9 cell lysate stained with ARG59633 anti-E-Ras antibody at 1:1000 dilution.
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ARG59633 anti-E-Ras antibody FACS image
Flow Cytometry: F9 cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% BSA to block non-specific protein-protein interactions followed by ARG59633 anti-E-Ras antibody (right histogram) at 1:25, 60 min at 37°C, followed by DyLight®488 labelled secondary antibody. Isotype control antibody (left histogram) was Rabbit IgG1 (1 µg/10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.
