ARG52270
anti-Dynamin phospho (Ser778) antibody
anti-Dynamin phospho (Ser778) antibody for Western blot and Rat
Neuroscience antibody; Signaling Transduction antibody
概述
| 产品描述 | Sheep Polyclonal antibody recognizes Dynamin phospho (Ser778) |
|---|---|
| 反应物种 | Rat |
| 预测物种 | Hu, Ms, Bov, Chk, Dog |
| 应用 | WB |
| 宿主 | Sheep |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Dynamin |
| 抗原物种 | Rat |
| 抗原 | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser778 conjugated to KLH |
| 偶联标记 | Un-conjugated |
| 別名 | DNM; EC 3.6.5.5; Dynamin-1; EIEE31 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | Specific for the ~95k dynamin protein phosphorylated at Ser778. Labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Does not cross react with other purified substRates of cdk5 (e.g. amphiphysin and synapsin). Immunolabeling is blocked by λ-phosphatase treatment. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | DNM1 |
| 全名 | dynamin 1 |
| 背景介绍 | Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001; Graham et al., 2002; Tsuboi et al., 2002). Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo (Tan et al., 2003). Phosphorylation of these sites on dynamin is thought to play a key role in synaptic vesicle trafficking. |
| 研究领域 | Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 97 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG52270 anti-Dynamin phospho (Ser778) antibody WB image
Western blot: Rat hippocampal lysate stimulated with forskolin then stained with ARG52270 anti-Dynamin phospho (Ser778) antibody showing specific immunolabeling of the ~95k dynamin phosphorylated at Ser778 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubated in λ-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser778 dynamin. The immunolabeling is completely eliminated by treatment with λ-Ptase.
