ARG41445

anti-Doublecortin antibody

anti-Doublecortin antibody for Flow cytometry,Western blot and Human,Rat

Controls and Markers antibody; Neuroscience antibody; Hippocampal Neurogenesis Marker antibody; Immature Neuronal Cells Marker antibody

概述

产品描述 Rabbit Polyclonal antibody recognizes Doublecortin
反应物种 Hu, Rat
应用 FACS, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Doublecortin
抗原物种 Human
抗原 Synthetic peptide of Human Doublecortin.
偶联标记 Un-conjugated
別名 LISX; Doublin; SCLH; Lissencephalin-X; Neuronal migration protein doublecortin; DC; Lis-X; DBCN; XLIS

应用说明

应用建议
应用 推荐稀释比
FACS1:50 - 1:200
WB1:500 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 C6
实际分子量 ~ 43 kDa

属性

形式 Liquid
纯化 Affinity purified.
缓冲液 PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 1641 Human DCX

Swiss-port # O43602 Human Neuronal migration protein doublecortin

基因名称 DCX
全名 doublecortin
背景介绍 This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, mental retardation, subcortical band heterotopia ("double cortex" syndrome) in females and lissencephaly ("smooth brain" syndrome) in males. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010]
生物功能 Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration. [UniProt]
细胞定位 Cytoplasm. Cell projection. Note=Localizes at neurite tips. [UniProt]
研究领域 Controls and Markers antibody; Neuroscience antibody; Hippocampal Neurogenesis Marker antibody; Immature Neuronal Cells Marker antibody
预测分子量 41 kDa
翻译后修饰 Phosphorylation by MARK1, MARK2 and PKA regulates its ability to bind microtubules (By similarity). Phosphorylation at Ser-265 and Ser-297 seems to occur only in neonatal brain, the levels falling precipitously by postnatal day 21 (By similarity).

Ubiquitinated by MDM2, leading to its degradation by the proteasome. Ubiquitinated by MDM2 and subsequent degradation leads to reduce the dendritic spine density of olfactory bulb granule cells. [UniProt]

检测图片 (1) Click the Picture to Zoom In

  • ARG41445 anti-Doublecortin antibody WB image

    Western blot: C6 cell lysate stained with ARG41445 anti-Doublecortin antibody.