ARG52266
anti-Doublecortin antibody [3 e1]
anti-Doublecortin antibody [3 e1] for ICC/IF,IHC-Frozen sections,Western blot and Bovine,Human,Mouse,Rat
Controls and Markers antibody; Neuroscience antibody; Hippocampal Neurogenesis Marker antibody; Immature Neuronal Cells Marker antibody
概述
| 产品描述 | Mouse Monoclonal antibody [3 e1] recognizes Doublecortin |
|---|---|
| 反应物种 | Hu, Ms, Rat, Bov |
| 应用 | ICC/IF, IHC-Fr, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 3 e1 |
| 同位型 | IgG2A |
| 靶点名称 | Doublecortin |
| 抗原物种 | Human |
| 抗原 | Full length recombinant human protein expressed in and purified from E. coli |
| 偶联标记 | Un-conjugated |
| 別名 | LISX; Doublin; SCLH; Lissencephalin-X; Neuronal migration protein doublecortin; DC; Lis-X; DBCN; XLIS |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | Specific for the ~35 & 45 k doublecortin protein. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS, 5 mM Sodium azide and 50% Glycerol. |
| 抗菌剂 | 5 mM Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O43602 Human Neuronal migration protein doublecortin Swiss-port # O88809 Mouse Neuronal migration protein doublecortin |
|---|---|
| 基因名称 | DCX |
| 全名 | doublecortin |
| 背景介绍 | Doublecortin, or DCX, is a microtubule associated protein that is expressed almost exclusively in very early neuronal development (Brown et al., 2003), making it an excellent marker for developing neuronal cells. Defects in the DCX gene lead to X-linked lissencephaly which is characterized by a lack of normal folds on the surface of the brain resulting in a smooth cerebral cortex caused by abnormal migration of neurons during development (des Portes et al., 1998; Gleeson et al., 1998). |
| 产品亮点 | Related Antibody Duos and Panels: ARG30093 Hippocampal Neurogenesis Marker Antibody Duo (Prox1, Dcx) Related products: Doublecortin antibodies; Doublecortin Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: "Pro-aging factor" tied to immune-related molecule Neuronal Development Marker |
| 研究领域 | Controls and Markers antibody; Neuroscience antibody; Hippocampal Neurogenesis Marker antibody; Immature Neuronal Cells Marker antibody |
| 预测分子量 | 41 kDa |
| 翻译后修饰 | Phosphorylation by MARK1, MARK2 and PKA regulates its ability to bind microtubules (By similarity). Phosphorylation at Ser-265 and Ser-297 seems to occur only in neonatal brain, the levels falling precipitously by postnatal day 21 (By similarity). Ubiquitinated by MDM2, leading to its degradation by the proteasome. Ubiquitinated by MDM2 and subsequent degradation leads to reduce the dendritic spine density of olfactory bulb granule cells. |
检测图片 (4) Click the Picture to Zoom In
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ARG52266 anti-Doublecortin antibody [3 e1] ICC/IF image
Immunofluorescence: cultured rat neurons showing strong cytoplasmic staining of doublecortin (green)(ARG52266) in developing neurons and GFAP (ARG52313) in red.
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ARG52266 anti-Doublecortin antibody [3 e1] ICC/IF image
Immunofluorescence: Cortical neuron-glial cell culture from E20 Rat stained with ARG52266 anti-Doublecortin antibody [3 e1] (red) at 1:1000 dilution, and costained with anti-MAP2 antibody (green) at 1:10000 dilution. DAPI (blue) for nuclear staining.
The Doublecortin antibody reveals strong cytoplasmic staining in a population of small developing neurons and their processes, while the MAP2 antibody stains dendrites and perikarya of mature neurons.
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ARG52266 anti-Doublecortin antibody [3 e1] WB image
Western Blot: postnatal day 3 rat brain lysate stained with Doublecortin antibody (ARG52266) showing specific immunolabeling of the ~35 & 45k doublecortin protein.
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ARG52266 anti-Doublecortin antibody [3 e1] WB image
Western blot: Adult Rat brain (negative control) and embryonic E20 Rat brain lysates stained with ARG52266 anti-Doublecortin antibody [3 e1] (green) at 1:1000 dilution.
