ARG42043
anti-Dopamine beta Hydroxylase antibody
anti-Dopamine beta Hydroxylase antibody for ICC/IF,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Dopamine beta Hydroxylase |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Dopamine beta Hydroxylase |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide between aa. 27-56 of Human Dopamine beta Hydroxylase. |
| 偶联标记 | Un-conjugated |
| 別名 | EC 1.14.17.1; DBM; Dopamine beta-monooxygenase; Dopamine beta-hydroxylase |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Rat adrenal gland | ||||||
| 实际分子量 | ~ 75 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | DBH |
| 全名 | dopamine beta-hydroxylase (dopamine beta-monooxygenase) |
| 背景介绍 | The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. [provided by RefSeq, Jul 2008] |
| 生物功能 | Conversion of dopamine to noradrenaline. [UniProt] |
| 细胞定位 | Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle, secretory vesicle lumen. Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen. Secreted. Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type II membrane protein. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Single-pass type II membrane protein. [UniProt] |
| 预测分子量 | 69 kDa |
| 翻译后修饰 | N-glycosylated. Proteolytic cleavage after the membrane-anchor leads to the release of the soluble form. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG42043 anti-Dopamine beta Hydroxylase antibody ICC/IF image
Immunofluorescence: 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells stained with ARG42043 anti-Dopamine beta Hydroxylase antibody (green) at 1:25 dilution. Dylight® 554 Phalloidin (red) for cytoplasmic actin staining. DAPI (blue) for nuclear staining.
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ARG42043 anti-Dopamine beta Hydroxylase antibody WB image
Western blot: 20 µg of Rat adrenal gland lysate stained with ARG42043 anti-Dopamine beta Hydroxylase antibody at 1:2000 dilution.
