ARG52265
anti-Dopamine Transporter phospho (Thr53) antibody
anti-Dopamine Transporter phospho (Thr53) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Rat
Neuroscience antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Dopamine Transporter phospho (Thr53) |
|---|---|
| 反应物种 | Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Dopamine Transporter |
| 抗原物种 | Rat |
| 抗原 | Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr53 conjugated to KLH |
| 偶联标记 | Un-conjugated |
| 別名 | PKDYS; Sodium-dependent dopamine transporter; Solute carrier family 6 member 3; DAT1; DAT; DA transporter |
应用说明
| 应用建议 |
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| 应用说明 | Specific for the ~55k glycosylated form of the DAT protein phosphorylated at Thr53. Relative mobility may vary depending on the state of glycosylation of the DAT protein. The antibody works best in lysates that have not been boiled prior to being run on an SDS-PAGE gel. Immunolabeling of the DAT band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P23977 Rat Sodium-dependent dopamine transporter |
|---|---|
| 基因名称 | SLC6A3 |
| 全名 | solute carrier family 6 (neurotransmitter transporter), member 3 |
| 背景介绍 | The dopamine transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996). It has been shown that phosphorylation at Thr53 directly affects dopamine influx and amphetamine-stimulated substrate efflux, indicating that the Thr53 residue plays a major role in transport activity (Foster et al., 2012). |
| 产品亮点 | Related Antibody Duos and Panels: ARG30102 Phospho Dopamine Transporter Antibody Duo (Total, pT53) Related products: Dopamine Transporter antibodies; Dopamine Transporter Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| 研究领域 | Neuroscience antibody |
| 预测分子量 | 68 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG52265 anti-Dopamine Transporter phospho (Thr53) antibody WB image
Western blot: Rat caudate lysate with (lane 2) or without (lane 1) phospho-peptide stained with ARG52265 anti-Dopamine Transporter phospho (Thr53) antibody.
