ARG22285
anti-Dnmt3L antibody [S117-9]
anti-Dnmt3L antibody [S117-9] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse
概述
| 产品描述 | Mouse Monoclonal antibody [S117-9] recognizes Dnmt3L |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ICC/IF, IHC-P, IP, WB |
| 特异性 | Detects ~45kDa. No cross-reactivity against DNMT3a or DNMT3B. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | S117-9 |
| 同位型 | IgG1 |
| 靶点名称 | Dnmt3L |
| 抗原物种 | Human |
| 抗原 | Fusion protein around aa. 1-387 of Human Dnmt3L |
| 偶联标记 | Un-conjugated |
| 別名 | DNA (cytosine-5)-methyltransferase 3-like |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9CWR8 Mouse DNA (cytosine-5)-methyltransferase 3-like Swiss-port # Q9UJW3 Human DNA (cytosine-5)-methyltransferase 3-like |
|---|---|
| 基因名称 | DNMT3L |
| 全名 | DNA (cytosine-5-)-methyltransferase 3-like |
| 背景介绍 | CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases, but is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, it does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012] |
| 生物功能 | Catalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4) and induces de novo DNA methylation by recruitment or activation of DNMT3. [UniProt] |
| 细胞定位 | Nucleus |
| 预测分子量 | 44 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG22285 anti-Dnmt3L antibody [S117-9] WB image
Western blot: Human tissue lysate stained with ARG22285 anti-Dnmt3L antibody [S117-9] at 1:1000 dilution.
