ARG64495
anti-Dnmt1 antibody
anti-Dnmt1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Gene Regulation antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes DNMT1 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow, Dog, Pig |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Dnmt1 |
| 抗原物种 | Human |
| 抗原 | C-RFESPPKTQPTEDN |
| 偶联标记 | Un-conjugated |
| 別名 | CXXC-type zinc finger protein 9; DNA; Dnmt1; cytosine-5; ADCADN; AIM; DNMT; M.HsaI; MCMT; HSN1E; EC 2.1.1.37; DNA methyltransferase HsaI; CXXC9; DNA MTase HsaI |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P26358 Human DNA (cytosine-5)-methyltransferase 1 |
|---|---|
| 背景介绍 | DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008] |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 183 kDa |
| 翻译后修饰 | Sumoylated; sumoylation increases activity. Acetylation on multiple lysines, mainly by KAT2B/PCAF, regulates cell cycle G(2)/M transition. Deacetylation of Lys-1349 and Lys-1415 by SIRT1 increases methyltransferase activity. Phosphorylation of Ser-154 by CDKs is important for enzymatic activity and protein stability. Phosphorylation of Ser-143 by AKT1 prevents methylation by SETD7 therebye increasing DNMT1 stability. Methylation at Lys-142 by SETD7 promotes DNMT1 proteasomal degradation. Ubiquitinated by UHRF1; interaction with USP7 counteracts ubiquitination by UHRF1 by promoting deubiquitination and preventing degradation by the proteasome. |
检测图片 (2) Click the Picture to Zoom In
-
ARG64495 anti-DNMT1 antibody WB image
Western Blot: Jurkat cell lysate (35 µg protein in RIPA buffer) stained with ARG64495 anti-DNMT1 antibody at 0.5 µg/ml dilution.
-
ARG64495 anti-DNMT1 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Kidney. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64495 anti-DNMT1 antibody at 3.8 µg/ml dilution followed by AP-staining.
