ARG55201
anti-Dnmt1 antibody
anti-Dnmt1 antibody for ChIP,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Gene Regulation antibody
1
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Dnmt1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ChIP, ICC/IF, IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Dnmt1 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein of Human Dnmt1 (Swiss: P26358) |
| 偶联标记 | Un-conjugated |
| 別名 | AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; DNA (cytosine-5)-methyltransferase 1; Dnmt1; EC 2.1.1.37; CXXC-type zinc finger protein 9; DNA methyltransferase HsaI; DNA MTase HsaI; M.HsaI; MCMT |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||||
| 阳性对照 | HeLa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P13864 Mouse DNA (cytosine-5)-methyltransferase 1 Swiss-port # P26358 Human DNA (cytosine-5)-methyltransferase 1 |
|---|---|
| 基因名称 | DNMT1 |
| 全名 | DNA (cytosine-5-)-methyltransferase 1 |
| 背景介绍 | DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008] |
| 生物功能 | Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. [UniProt] |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 183 kDa |
| 翻译后修饰 | Sumoylated; sumoylation increases activity. Acetylation on multiple lysines, mainly by KAT2B/PCAF, regulates cell cycle G(2)/M transition. Deacetylation of Lys-1349 and Lys-1415 by SIRT1 increases methyltransferase activity. Phosphorylation of Ser-154 by CDKs is important for enzymatic activity and protein stability. Phosphorylation of Ser-143 by AKT1 prevents methylation by SETD7 therebye increasing DNMT1 stability. Methylation at Lys-142 by SETD7 promotes DNMT1 proteasomal degradation. Ubiquitinated by UHRF1; interaction with USP7 counteracts ubiquitination by UHRF1 by promoting deubiquitination and preventing degradation by the proteasome. |
检测图片 (2) Click the Picture to Zoom In
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ARG55201 anti-Dnmt1 antibody WB image
Western blot: 20 µg and 10 µg of HeLa cell lysates stained with ARG55201 anti-Dnmt1 antibody at 1:500 dilution.
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ARG55201 anti-Dnmt1 antibody ICC/IF image
Immunofluorescence: A549 cells stained with ARG55201 anti-Dnmt1 antibody.
客户反馈
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anti-Dnmt1 antibody
Application：WB
Sample：HeLa
Sample Loading Amount：20 µg (left), 10 µg (right)
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
