ARG20541
anti-Dnmt1 antibody [60B1220.1]
anti-Dnmt1 antibody [60B1220.1] for ChIP,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Fish,Zebrafish
Gene Regulation antibody
概述
| 产品描述 | Mouse Monoclonal antibody [60B1220.1] recognizes Dnmt1 |
|---|---|
| 反应物种 | Hu, Ms, Fsh, Zfsh |
| 应用 | ChIP, IHC-P, IP, WB |
| 特异性 | Cross-react with mouse DNMT1. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 60B1220.1 |
| 同位型 | IgG1, kappa |
| 靶点名称 | Dnmt1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around aa. 637-650 of Human DNMT1. |
| 偶联标记 | Un-conjugated |
| 別名 | AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; DNA (cytosine-5)-methyltransferase 1; Dnmt1; EC 2.1.1.37; CXXC-type zinc finger protein 9; DNA methyltransferase HsaI; DNA MTase HsaI; M.HsaI; MCMT |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS, 0.05% BSA and 0.05% Sodium azide |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 0.05% BSA |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P13864 Mouse DNA (cytosine-5)-methyltransferase 1 Swiss-port # P26358 Human DNA (cytosine-5)-methyltransferase 1 |
|---|---|
| 基因名称 | DNMT1 |
| 全名 | DNA (cytosine-5-)-methyltransferase 1 |
| 背景介绍 | Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells. |
| 生物功能 | Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. [UniProt] |
| 细胞定位 | Nucleus |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 183 kDa |
| 翻译后修饰 | Sumoylated; sumoylation increases activity. Acetylation on multiple lysines, mainly by KAT2B/PCAF, regulates cell cycle G(2)/M transition. Deacetylation of Lys-1349 and Lys-1415 by SIRT1 increases methyltransferase activity. Phosphorylation of Ser-154 by CDKs is important for enzymatic activity and protein stability. Phosphorylation of Ser-143 by AKT1 prevents methylation by SETD7 therebye increasing DNMT1 stability. Methylation at Lys-142 by SETD7 promotes DNMT1 proteasomal degradation. Ubiquitinated by UHRF1; interaction with USP7 counteracts ubiquitination by UHRF1 by promoting deubiquitination and preventing degradation by the proteasome. |
检测图片 (3) Click the Picture to Zoom In
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ARG20541 anti-Dnmt1 antibody [60B1220.1] IHC image
Immunohistochemistry: Medullar kidney tissue sections stained with ARG20541 anti-Dnmt1 antibody [60B1220.1].
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ARG20541 anti-Dnmt1 antibody [60B1220.1] WB image
Western blot: Human H1299 cell lysate stained with ARG20541 anti-Dnmt1 antibody [60B1220.1] at 1:1000 dilution.
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ARG20541 anti-Dnmt1 antibody [60B1220.1] IHC image
Immunohistochemistry: Formalin-fixed Human colon carcinoma stained with ARG20541 anti-Dnmt1 antibody [60B1220.1] at 1:10,000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x.
